Figure 6.

Affinity-matured nanobodies display high stability and specificity

(A) Melting temperatures of bivalent nanobodies and antibodies evaluated via differential scanning fluorimetry.

(B) Non-specific binding of bivalent nanobodies and antibodies (immobilized on magnetic beads) was evaluated via incubation with biotinylated soluble membrane proteins from CHO cells and detection of non-specific binding via flow cytometry. Control antibodies with high (emibetuzumab) and low (elotuzumab) non-specific binding were also evaluated for comparison. The two control antibodies are not identical to the actual drugs, as they have the variable regions of the actual drugs and a common IgG1 framework. In (A) and (B), the results are averages from three independent repeats, and the error bars are standard deviations.