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. 2021 Jun 24;28(9):1379–1388.e7. doi: 10.1016/j.chembiol.2021.05.019

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Nanobodies with nanomolar monovalent affinities can be generated via CDR-swapping mutagenesis without the need for lead clone identification and subsequent affinity maturation

Nanobodies were selected from a non-immune library with (KA1.ep1, K7.13, K7.19) or without (KC3, KA1, KC1) CDR-swapping mutagenesis, and the selected clones were evaluated in terms of their monovalent binding affinities for the SARS-CoV-2 receptor-binding domain. The results are averages from two independent experiments, and the error bars are standard deviations.