REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Emibetuzumab	Jain et al., 2017	N/A
Elotuzumab	Jain et al., 2017	N/A
Mouse anti-Myc-Tag antibody (9B11)	Cell Signaling Technology	Cat#2276S; RRID: AB_331783
Chicken anti-His-Tag antibody	Invitrogen	Cat#PA1-9531; RRID: AB_1069887
Goat anti-mouse IgG AlexaFluor 488	Invitrogen	Cat#A11001; RRID: AB_2534069
Donkey anti-chicken IgY F(ab’)2 AlexaFluor 647	Jackson ImmunoResearch	Cat#703-606-155; RRID: AB_2340380
Goat anti-human IgG AlexaFluor 647	Jackson ImmunoResearch	Cat#109-605-098; RRID: AB_2337889
Goat anti-human Fc F(ab’)2 AlexaFluor 488	Invitrogen	Cat#H10120; RRID: AB_2536548
Bacterial and virus strains
DH5α	Julian et al., 2017	N/A
SARS-Related Coronavirus 2, Isolate USA-WA1/2020	BEI	Cat#NR-52281
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (RBD-Fc)	Acro Biosystems	Cat#SPD-C5255
Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His, Avitag (bRBD)	Acro Biosystems	Cat#SPD-C82E9
Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His, Avitag	Acro Biosystems	Cat#S1N-C82E8
SARS-CoV S protein RBD, His Tag	Acro Biosystems	Cat#SPD-S52H6
SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer	Acro Biosystems	Cat#SPN-C52H8
Recombinant Human Angiotensin-converting Enzyme 2 (ACE2)	RayBiotech	Cat#230-30165
Taq DNA Polymerase with Standard Taq Buffer	New England Biolabs	Cat#M0273L
8-Oxo-2'-deoxyguanosine-5'-Triphosphate	TriLink Biotechnologies	Cat#N-2034
2’-Deoxy-P-nucleoside-5'-Triphosphate	TriLink Biotechnologies	Cat#N-2037
Q5 High-Fidelity DNA Polymerase	New England Biolabs	Cat#M0491L
NheI-HF	New England Biolabs	Cat#R3131L
HindIII-HF	New England Biolabs	Cat#R3104L
Quick CIP calf intestinal alkaline phosphatase	New England Biolabs	Cat#M0525L
T4 ligase	New England Biolabs	Cat#M0202L
F17 media	Thermo Fisher Scientific	Cat#A1383502
Glutamine	Invitrogen	Cat#25030081
Kolliphor	Fisher Scientific	Cat#NC0917244
G418	Thermo Fisher Scientific	Cat#10131035
Yeastolate	BD Biosciences	Cat#292804
Luciferase substrate	Promega ONE-Glo	Cat#E6110
Streptavidin AlexaFluor 647	Invitrogen	Cat#S32357
Protein Thermal Shift Dye	Applied Biosystems	Cat#4461146
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail	Sigma Aldrich	Cat#4693159001
cOmplete, EDTA-free Protease Inhibitor Cocktail	Sigma Aldrich	Cat#11873580001
1% n-dodecyl-b-D-maltopyranoside	Sigma Aldrich	Cat#D4641
Sulfo-NHS-LC-biotin	Thermo Fisher Scientific	Cat#21335
Critical commercial assays
DC Protein Assay Reagents Package	BioRad	Cat#5000116
Deposited data
KA1	This paper	GenBank: MZ133804
KC3	This paper	GenBank: MZ133805
KC1	This paper	GenBank: MZ133806
KA1.ep1	This paper	GenBank: MZ133807
KC3.ep3	This paper	GenBank: MZ133808
KC3.ep5	This paper	GenBank: MZ133809
K7.13	This paper	GenBank: MZ133810
K7.19	This paper	GenBank: MZ133811
Ty1	Hanke et al., 2020	PDB: 6ZXN
VHH-72	Wrapp et al., 2020	PDB: 6WAQ
CB6	Shi et al., 2020	PDB: 7C01
C119	Barnes et al., 2020 Provided by laboratories of Pamela Bjorkman (California Institute of Technology) and Michel Nussenzweig (The Rockefeller University)	PDB: 7K8W
S309	Pinto et al., 2020 Provided by laboratories of Pamela (California Institute of Technology) Bjorkman and Michel Nussenzweig (The Rockefeller University)	PDB: 6WPS
CR3022	Yuan et al.	PDB: 6W41
Experimental models: Cell lines
Human: HEK293-6E	National Research Council (NRC) of Canada	N/A
Human: Lenti-X 293T	Takara	Cat#632180
Human: 293T-ACE2	BEI	Cat#NR-52511
Cercopithecus aethiops: VeroE6	ATCC	Cat#CRL1586
Hamster: CHO	Gibco	Cat#A29133
Yeast: EBY100	Julian et al. 2017	N/A
Recombinant DNA
HDM-Hgpm2 plasmid encoding HIV Gag-Pol under CMV promoter	BEI	Cat#NR-52517
HDM-tat1b plasmid encoding HIV Tat under CMV promoter	BEI	Cat#NR-52518
pRC14 CMV-Rev1b plasmid encoding HIV Rev	BEI	Cat#NR-52519
pHAGE-CMV-Luc2-IRES-ZsGreen-W lentiviral transfer plasmid encoding co-expression of luciferase and ZsGreen	BEI	Cat#NR-52516
pCMV3 SARS-CoV2 S Untagged Delta 19AA C-term plasmid encoding the SARS-CoV-2 spike (S) protein with a 19-amino acid deletion at the C-terminus	Provided by the laboratory of Marilia Cascalho (University of Michigan)	N/A
Software and algorithms
Python version 3.7	Python Software Foundation	https://www.python.org
Code for curve fitting	This paper	https://GitHub.com/mduncans/Data_Fitting
Others
Synthetic nanobody library	McMahon et al., 2018 Provided by the laboratory of Andrew Kruse (Harvard Medical School)	N/A
Streptavidin MicroBeads	Miltenyi Biotec	Cat#130-048-101
Zymoprep Yeast Plasmid Miniprep II	Zymo Research	Cat#D2004
Streptavidin Dynabeads	Invitrogen	Cat#11047
Protein A magnetic beads	Invitrogen	Cat#88846
QIAquick Gel Extraction Kit	Qiagen	Cat#28704
QIAquick PCR Purification Kit	Qiagen	Cat#28104
QIAprep Spin Miniprep Kit	Qiagen	Cat#27106
Protein A Agarose	Thermo Fisher Scientific	Cat#20333
Centrifuge columns	Thermo Fisher Scientific	Cat#89898
Zeba Spin Desalting Columns	Thermo Fisher Scientific	Cat#89894; Cat#89892; Cat#89890
Invitrogen NuPAGE 10% Bis-Tris, 1.0 mm, Midi Protein GEL (SDS-PAGE)	Thermo Fisher Scientific	Cat#WG1203BOX
Lenti-X Concentrator	Takara	Cat#631232
Superdex 200 Increase 10/300 GL column	GE	Cat#28990944
96-well plates	VWR	Cat#650261