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. 2021 Jun 1;15(6):9600–9613. doi: 10.1021/acsnano.0c09958

Figure 4.

Figure 4

Peptide recognition of (mutant) fragaceatoxin C. Mutations are shown in red on the lumen of fragaceatoxin C modeled on PDB: 4TSY. The fit of the residual current is shown for angiotensin IV (VYIHPF), angiotensin III (RVYIHPF), angiotensin I (DRVYIHPFHL), and angiotensinogen (DRVYIHPFHLVIHN) each in 2.5 μM concentration, recorded under an applied potential of −50 mV. Traces were collected in 1 M KCl and 50 mM citric acid titrated with bis-tris propane to pH 3.8 at a sampling frequency of 50 kHz, using a 10 kHz Bessel filter and 5 kHz Gaussian filter. Histograms were created from all events with a dwell time larger than 200 μs, with the exception of G13F, G13Y, and G13W, where the minimal dwell time was set to 1 ms. The dwell time set against the residual current shows all events with a dwell time larger than 200 μs. The marker size of data points in the residual current set against the dwell time is adjusted for visualization.