Table 1.

Primer sequences and amplification programs.

Gene	Primers sequence	Conditions	Product size (bp)	Ref
HBD-2	5’-CCAGCCATCAGCCATGAGGGT-3’ 5’- GGAGCCCTTTCTGAATCCGCA-3’	10’’at 95°C, 5”at 60°C, 10” for 40 cycles	254	Fusco et al. (2017)
HBD-3	5’- CGGCAGCATTTTGCGCCA-3’ 5’- CTAGCAGCTATGAGGATC-3’	10’’at 95°C, 4”at 58°C, 8” for 40 cycles	206	Fusco et al. (2017)
Occludin	5’-TCAGGGAATATCCACCTATCACTTCAG-3’ 5’-CATCAGCAGCAGCCATGTACTCTTCAC-3’	10’’at 95°C, 45”at 60°C for 40 cycles	188	Cuffaro et al. (2020)
Zonulin-1	5’-AGGGGCAGTGGTGGTTTTCTGTTCTTTC-3’ 5’-GCAGAGGTCAAAGTTCAAGGCTCAAGAGG-3’	10’’at 95°C, 45”at 60°C for 40 cycles	217	Wilms et al. (2019)
Claudin-1	5’-CTGGGAGGTGCCCTACTTTG-3’ 5’-ACACGTAGTCTTTCCCGCTG-3’	1’’at 95°C, 30” at 60°C, 20’’at 72°C for 40 cycles	128	Wu et al. (2018)
MUC-2	5’-CTGCACCAAGACCGTCCTCATG-3’ 5’-GCAAGGACTGAACAAAGACTCAGAC-3’	5’’at 96°C, 16” at 60°C, 8’’at 72°C for 40 cycles	401	Perrais et al. (2002)
Bcl-2	5’-CAGCTGCACCTGACGCCCTT-3’ 5’-CCCAGCCTCCGTTATCCTGGA-3’	5’’at 94°C, 7” at 58°C, 9’’at 72°C for 40 cycles	235	Fusco et al. (2018)
Fas-L	5’-GGATTGGGCCTGGGGATGTTTCA-3’ 5’-TTGTGGCTCAGGGGCAGGTTGTTG-3’	5’’at 95°C, 7” at 60°C, 14’’at 72°C for 40 cycles	344	Fusco et al. (2018)
Fas-R	5’- CCAAGTGACTGACATCAACTC-3’ 5’- CTCTTTGCACTTGGTGTTGCTGG -3’	5”at 94°C, 8”at 55°C, 17”at 72°C for 40 cycles	426	Fusco et al. (2018)
β-actin	5’-GACGACGACAAGATAGCCTAGCAGCTATGAGGATC-3’ 5’-GAGGAGAAGCCCGGTTAACTTCCGCAGCATTTTGCGCCA-3’		243	Fusco et al. (2018)