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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Cancer. 2021 Apr 1;2(4):414–428. doi: 10.1038/s43018-021-00183-y

Extended Data Fig. 8. FASN knockdown alters cell lipid composition.

Extended Data Fig. 8

a) The indicated acylglycerol levels measured by LCMS from control and FASN-disrupted (sgFASN_1) BT474 tumors growing in the brain of NSG mice are presented as a ratio to show how levels differ based on FASN expression. A black dotted line indicates a ratio of 1, representing no difference in lipid levels between sgFASN_1 and control BT474 tumor tissue. * q < 0.1 by Multiple t-test, False Discovery Rate corrected (n=4 brain tumors).

b) Complex lipid levels measured by LCMS from control and FASN-disrupted (sgFASN_1) BT474 cells in culture (+Lipids) are presented as a ratio to show how levels differ based on FASN expression. A black dotted line indicates a ratio of 1, representing no difference in lipid levels between sgFASN_1 and control cells. Lipid levels were normalized to protein content as determined by sulforhodamine B. All comparisons are significant, q < 0.1 by Multiple t-test, False Discovery Rate corrected. (n=3 cell culture biological replicates).