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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Cancer. 2021 Apr 1;2(4):414–428. doi: 10.1038/s43018-021-00183-y

Extended Data Fig. 10. Lipid availability determines response to pharmacological FASN inhibition.

Extended Data Fig. 10

a-c) BT474 brain tumor-derived organotypic slice cultures were treated for 6 days with vehicle or 500 nM TVB3166 in standard (+Lipids) or delipidated (-Lipids) media. The percent of apoptotic and viable HER2 positive BT474 cells was determined by measuring Annexin V (AV) and 7-amino actinomycin D (7AAD) uptake by flow cytometry. The flow cytometry gating strategy is presented in Supplementary Figure 2. * p = 0.0111, *** p = 0.0003 by one-way ANOVA followed by Tukeýs test (n=5 BT474 brain tumor slices).

d,e) Mouse weights for the cohort presented in Figure 6f,g that was treated daily with BI99179 (15mg/kg) or vehicle control by oral gavage (n=5 mice).

Data presented in all panels are means ± SEM.