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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: FASEB J. 2021 Apr;35(4):e21437. doi: 10.1096/fj.202002755R

Figure 1. Determining basal CaMKII isoform expression in human and murine endothelium.

Figure 1.

A. qPCR analysis of CaMKII subtypes CAMK2A, CAMK2B, CAMK2D, and CAMK2G in cultured HUVEC normalized to GAPDH. n = 3. B. Diagram of custom CaMKII antibody binding sites on CaMKIIδ and γ recognizing unique splice isoforms. Immunoblot for the variable domain of γ and the C-terminal tail of CaMKIIδ reveals differential isoform expression in endothelial cells when compared to rat vascular smooth muscle cells (rVSM). n = 3.