Figure 6.

NP-specific CD8+ T-cells from OVX836-immunized mice are cytotoxic effector cells. (A) Splenocytes from naive C57/B6 mice were labelled with CFSE: the CFSE^low peak corresponds to NP_366–377-pulsed target cells, the CFSE^high peak corresponds to unpulsed target cells. CFSE target cells were mixed at a 1:1 ratio and incubated for 16 h with sorted CD8+ T-cells isolated from the lungs and spleens of OVX836-vaccinated mice to measure antigen-specific CD8+ T-cell-mediated killing. (B) The data are representative of two separate experiments yielding similar results, represented in box-and-whisker plots (min and max, all points are shown) in the right panel. Differences were assessed by one-way ANOVA followed by Tukey’s multiple comparison test. Significance was set at ***p < 0.001, ****p < 0.0001.