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. 2021 Jun 10;12:678483. doi: 10.3389/fimmu.2021.678483

Figure 7.

Figure 7

NP-specific CD8+ T-cells protect mice from lethal influenza challenges. (A, B) Donor C57BL/6 mice (n = 6–7 in each group) received two IM immunizations (D0, D21) with OVX836 (30 μg), buffer (control mice), or OVA (10 μg) + IFA. Spleens and/or lungs were collected from the donor mice at D28 to obtain CD8+ T-cells for the transfer (A). Lungs to obtain CD8+ T-cells and serum were collected from the donor mice at D36 for the transfer (B). Naive C57BL/6 recipient mice (n = six in each group) received 5 × 105 lung-enriched CD8+ T-cells by the IV route or 300 μl of serum by the intraperitoneal (IP) route. Some 24 h after this adoptive transfer, all recipient mice were IN infected with 104.7 TCID50/20 µl of the influenza viral strain H1N1 A/California/07/2009 (A) or H1N1 A/WSN/33 (B). OVX836-vaccinated mice were used as positive controls in each experiment. Mice were then observed daily for clinical signs and body weight changes for 10 days. Percent survival rates are presented. *p < 0.05, **p < 0.01 by Log-Rank (Mantel–Cox) test.