Table 1.
Structural characteristics of the NP proteins.
| Construct | Mass (Da)+ | DH (nm)* | EM** | Length (nm)** | Ti (°C)++ |
|---|---|---|---|---|---|
| NPm | 56103.77 | 6.5 ± 0.3 | Monomer | n/d | 52.7 |
| NPwt | 56246.89 | 11.2 ± 0.3 | Trimer | 13 ± 2 | 75.1 |
| Heptamer to oligo-heptamer | 18 ± 2 (heptamer) | ||||
| OVX836 | 62710.56 | 41.0 ± 1.4 | 43 ± 16 (di-heptamer) | 76.0 |
The observed average masses of recombinant proteins by mass spectrometry agree with theoretical primary structures (+). Hydrodynamic diameters (DH) obtained from scattering intensity DLS distributions (*). Main species and lengths seen in electron micrographs (**). Inflection temperatures (Ti) obtained from melting curves by nDSF measurements (++).