Skip to main content
. 2021 Jun 10;12:653819. doi: 10.3389/fendo.2021.653819

Figure 3.

Figure 3

The effects of RBP4 on glucose uptake and phosphorylation of IRS/PI3K/Akt in UA-induced 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with UA (10 mg/dl), RBP4 siRNA (50nM), or UA+RBP4 siRNA for 48h. (A) About 30 μg protein were loaded and the expression of RBP4 was examined by western blot. The representative views are shown in the left panel and densitrometric quantification analysis for RBP4 is shown in the right panel. (B) The mRNA expression of RBP4 was determined by RT-PCR. (C) Glucose uptake was determined by a glucose test kit. (D) About 30 μg protein were loaded and the expression of p-IRS-1 (PY896), p-IRS-2 (S731), p-PI3K (P85), and p-Akt (Ser473) were determined by western blot. The representative views are shown in the left panel and densitrometric quantification analysis for RBP4 is shown in the right panel. Results are represented by mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. NS, not significant. All the experiments were repeated three times.