FIG 2.
RSV NP and RdRpopt are required for MR3(−)eGFP expression in N. benthamiana leaves. (A) N. benthamiana leaves were infiltrated with mixed Agrobacterium cultures carrying MR3(−)eGFP and four VSRs (NSs and P19-HcPro-γb) with empty vector (Vec), NP, RdRpopt, or both NP and RdRpopt. The infiltrated leaves were examined and photographed under an inverted fluorescence microscope at 5 dpi. Bars, 200 μm. (B) Western blot analyses using the samples described for panel A and an NP- or eGFP-specific antibody. The Ponceau S-stained RubisCO large-subunit gel was used to show sample loadings. (C) Northern blot analyses using the samples described for panel A and a DIG-labeled sense or an antisense eGFP probe. The red and blue arrowheads indicate the antigenomic and genomic RNA3 expressed in the infiltrated leaves, respectively. An ethidium bromide-stained rRNA gel was used to show sample loadings.