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RNA secondary structures of the 5′ UTR, NS3 ORF, and IGR of RSV RNA3 segment. Secondary structure was predicted using the RNAfold web server (http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi) based on a thermodynamic prediction of minimal free energy (MFE). Red, black, and blue arrows indicate the nucleotide positions in the 5′ UTR, NS3, and IGR regions, respectively. Dotted boxes indicate that hairpins 1 and 2 were formed by the IGR, NS3, and 5′ UTR of RSV RNA3.
