TABLE 1.
RSV NSvc4 enhances cell-to-cell movement of the MR3(−)eGFP mini-replicon in the presence of NP, RdRpopt, and four VSRs
| NSvc4a OD600 | Total no. of eGFP fluorescent cells | No. of clustersb (% of total) with |
||
|---|---|---|---|---|
| 1 cell | 2 cells | ≥3 cells | ||
| 0 | 18 | 18 (100) | 0 (0) | 0 (0) |
| 0.025 | 54 | 15 (27.8) | 6 (22.2) | 5 (50) |
| 0.05 | 76 | 12 (15.8) | 7 (18.4) | 10 (65.8) |
| 0.1 | 95 | 8 (8.4) | 11 (23.2) | 13 (68.4) |
| 0.15 | 140 | 17 (12.1) | 9 (12.9) | 18 (75) |
a
Agrobacterium cultures harboring the plasmid encoding NSvc4 (final concentrations [OD600], 0, 0.025, 0.05, 0.1, and 0.15) were used to infiltrate N. benthamiana leaves.
b
eGFP fluorescent foci of RSV MR3(−)eGFP at 5 dpi.