FIG 9.

RNA transcription, protein expression, genome replication, and virion and SVP secretion from prcccDNA constructs. Huh7 cells were transfected with the prcccDNA constructs and harvested 3 days later for RNA analysis (A) or 5 days later for DNA and protein analysis (B to F). (A) Northern blot analysis of intracellular HBV RNAs using the 0.7-kb probe. (B and C) Southern blot analysis of intracellular replicative DNA (B) and extracellular virion DNA (C) using the full-length probe. (D) Western blot analysis of intracellular L, S, HBx, and core proteins using GAPDH as a loading control. (E) Western blot analysis of secreted L and S proteins following PEG precipitation of virions and SVPs. (F) ELISA of secreted HBsAg and HBeAg. Data were averaged from three transfection experiments, with the value for the WT construct set as 100%.