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. 2021 May 21;10(6):1274. doi: 10.3390/cells10061274

Figure 4.

Figure 4

Protein expression profile of the LOs and BLOs groups. (A) Immunostaining of BLOs and LOs derived from hESCs for AFP, ALB, CYP3A4, CYP1A2, ZO-1, E-cadherin, and vimentin/CD31. The nuclei were counterstained with DAPI (scale bar: 200 and 100 μm). (B) The ratios of ALB+, CYP3A4+, ALB/CYP3A4+, CYP1A2+, ZO-1+, E-cadherin+, vimentin+, and CD31+ cells in all DAPI-stained cells obtained from at least 4 views per section for at least 3 sections * p < 0.05 and ** p < 0.01. LOs: liver organoids; BLOs: bioengineered liver organoids.