Figure 6.

Cytotoxicity assay of the native and decellularized bovine nerves.

The effect of decellularized bovine nerves on primary rat adipose-derived stem cells was evaluated after 48 hours by MTT assay. Decellularized nerves after washing did not show any significant adverse effects (P > 0.05). Due to residual detergents, non-washed decellularized nerves had a negative effect on cell viability. The experiment was repeated at least three times. The optical densities were compared among the three groups by 4’,6-diamidino-2-phenylindole (n = 12). *P < 0.05, vs. native group; ^#P < 0.05, vs. washed group. Data are shown as the mean ± SD.