Figure 6.

Parasite load (number of amastigotes/100 cells) (A) and chemokine levels (pg/ml) (B–E) in L. (V.) braziliensis promastigote-infected THP-1 cells transiently transfected with miR-548d-3p inhibitor at 6 and 24 h post-infection. One experiment was carried out by adding the miR-548d-3p inhibitor (10 nM) or negative control (scrambled miRNA; 10 nM) with the transfection reagent diluted in RPMI medium or only RPMI medium (non-transfected cells) to wells containing 10⁶ THP-1 adherent cells and maintained for 24 h at 37°C (5% CO2) then infected with L. (V.) braziliensis promastigotes. (A) * = P < 0.05 (one way ANOVA and Student t test). MCP1/CCL2 (B), RANTES/CXCL5 (C), IL-8/CXCL8 (D), and IP-10/CXCL10 (E) concentrations were measured by flow-cytometry using the CBA kit. (B–E) * = p <0.05 (Kruskal-Wallis and Bonferroni tests).