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. 2021 Jun 23;41(7):e00016-21. doi: 10.1128/MCB.00016-21

FIG 3.

FIG 3

miR-181a-2-3p inhibition aggravated LPS-induced apoptosis and inflammatory response. (A) qRT-PCR was used to detect miR-181a-2-3p expression in miR-181a-2-3p inhibitor-transfected TCMK-1 cells. (B) Cell viability was detected using CCK-8 assay in each group. (C and D) Cell apoptosis was detected using flow cytometry analysis in each group. (E) Bax, Bcl-2, and C CASP3/-9 expression were determined using Western blotting. (F and G) qRT-PCR was performed to examine the levels of Bax, Bcl-2, IL-1β, IL-6, and TNF-α in each group. **, P < 0.01; ##, P < 0.01; ^^, P < 0.01.