FIG 5.

GJB2 expression was increased in LPS-induced cell model and CLP mice models. (A) The expression of GJB2 in sepsis mice and sham mice was measured by qRT-PCR. RT-PCR assay was used to detect GJB2 expression in siRNA-GJB2 transfection group (B) and vector expressing GJB2 transfection group (C). (D and I) The photomicrographs of kidney tissues stained by H&E (200×). (E, F, G, J, K, and L) ELISA was performed to examine the levels of Cr, BUN, NGAL, KIM-1, IL-1β, IL-6, and TNF-α in the serum of each group. (H and M) ELISA was performed to examine the caspase-3 activity. **, P < 0.01; ##, P < 0.01; ^^, P < 0.01.