FIG 1.

Loss of vigilin decreases cell survival postirradiation. (A) Tenfold serial dilution plating assays of wild-type (Wt) and vgl1Δ S. pombe cells showing bleomycin sensitivity. (B) Cell survival assay of wild-type and vgl1Δ S. pombe cells grown in the presence of bleomycin. (C) Ectopic expression of Vgl1 in cells with Vgl1 deleted rescues bleomycin sensitivity. (D) Western blot showing the knockdown of vigilin in HeLa and H1299 cell lines using vigilin-specific and control siRNAs. (E to G) Clonogenic cell survival assays after irradiation with different doses of IR in HeLa and H1299 cells with and without knockdown of vigilin. The error bars represent the standard deviations from three independent experiments.