FIG 6.

Ectopic expression of vigilin rescues DNA damage repair in vigilin-depleted cells. (A) Clonogenic survival after irradiation of H1299 cells with 4 Gy of IR with and without knockdown of endogenous vigilin and ectopic expression of vigilin. Complementation of vigilin in vigilin-deficient cells rescued cell survival postirradiation. (B) Homologous recombination frequencies in cells are shown after induction of double-strand breaks by I-SecI with and without knockdown of endogenous vigilin and ectopic expression of vigilin. Ectopic vigilin expression in vigilin knockdown cells restores survival and homologous recombination efficiency. (C) Chromatin immunoprecipitation analysis of Rad51 occupancy at the I-SecI site in cells with and without the depletion of vigilin and ectopic expression of vigilin. Ectopic expression of vigilin restores Rad51 occupancy to double-strand breaks in vigilin-deficient cells. The error bars in all experiments represent the standard deviations from three independent experiments.