FIG 8.

Vigilin-depleted cells have defective resolution of stalled DNA replication forks and firing of new origins of replication. (A) Schematic representation of the assay for DNA labeling and HU treatment for the fiber assay. (B) Representative images of new DNA replication forks and reinitiation of stalled DNA replication forks in control and vigilin-depleted cells. (C) Percentage of stalled DNA replication forks in control and vigilin-depleted cells after HU treatment. (D) DNA fiber analysis of IdU track lengths of CldU- plus IdU-containing fibers after hydroxyurea (HU) treatment. Error bars represent standard deviations from three independent experiments.