FIG 6.
Swi11–38 no longer forms high-molecular-weight, SDS-resistant aggregates when both phenylalanine residues are replaced. (A) Blot of SDD-AGE of BY4741 swi1Δ/p415TEF-SWI11–38Mut-YFP cells with (+) or without (−) boiling. The membrane was probed with anti-GFP to detect Swi11–38Mut-YFP. (B) Blot of SDD-AGE of W303 sup35Δ/p415TEF-SWI11–38Mut-MC/p416TEF-SWI11–38Mut-YFP cells with (+) or without (−) boiling and W303 sup35Δ/p316SUP35FL cells. The membrane was probed with anti-Sup35 to detect Swi11–38Mut-MC or Sup35FL.