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. 2021 Apr 27;372(6545):941–948. doi: 10.1126/science.abe7106

Fig. 5. LEOPARD with RNA preamplification and Bioanalyzer readout allows for multiplexed detection of SARS-CoV-2 in patient samples.

Fig. 5

(A) General workflow for LEOPARD with target-specific preamplification and DNA target resolution on a Bioanalyzer. (B) Sensitivity of the workflow for detecting dilutions of an in vitro–transcribed WT SARS-CoV-2 RNA fragment. One microliter was added for each test. Bars represent the average of independent duplicates. (C) Multiplexed detection of five RNAs in patient samples confirmed positive or negative for SARS-CoV-2 by RT-qPCR. (D) Sanger sequencing results of the detected region in SARS-CoV-2 cDNA from the positive patient samples. Blue bar: Position of the ncrRNA, with the thick part indicating the resulting ncrRNA portion.