Apoptosis analysis (immunoblotting). (A) Nuclear levels of p53 protein were evaluated in DLD1 after 3 h of 6 µg/mL of CCDP, 6 mg/mL of NK600, and Combo 6 exposure. The bars are expressed as % vs. untreated (U.T.) DLD1 cultures. (B) The figures depicted are representative of at least three similar immunoblot analysis p53 protein levels in untreated DLD1, and in treated DLD1 (6 µg/mL of CCDP, 6 mg/mL of NK600, and Combo 6). HDAC1 was used as internal controls for equal protein loading on gels. The data represent the mean ± standard deviation (SD) of 3 independent experiments. * treated DLD1 vs. U.T. DLD1; §CCDP vs. NK600/Combo6; @NK600 vs. CCDP/Combo 6. * p < 0.01; §
p < 0.01; @
p < 0.01 (two-way ANOVA followed by Bonferroni’s test).