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. 2021 May 21;11(6):1365. doi: 10.3390/nano11061365

Figure 5.

Figure 5

Apoptosis analysis (immunoblotting). (A) Nuclear levels of p53 protein were evaluated in DLD1 after 3 h of 6 µg/mL of CCDP, 6 mg/mL of NK600, and Combo 6 exposure. The bars are expressed as % vs. untreated (U.T.) DLD1 cultures. (B) The figures depicted are representative of at least three similar immunoblot analysis p53 protein levels in untreated DLD1, and in treated DLD1 (6 µg/mL of CCDP, 6 mg/mL of NK600, and Combo 6). HDAC1 was used as internal controls for equal protein loading on gels. The data represent the mean ± standard deviation (SD) of 3 independent experiments. * treated DLD1 vs. U.T. DLD1; §CCDP vs. NK600/Combo6; @NK600 vs. CCDP/Combo 6. * p < 0.01; § p < 0.01; @ p < 0.01 (two-way ANOVA followed by Bonferroni’s test).