. 2021 May 21;12(6):791. doi: 10.3390/genes12060791

Table 1.

Twelve candidate reference genes and primer sequences used in this study.

Gene Symbol	Gene Name	Primer Sequence (5′ to 3′)	Amplicon Size (bp)	PCR Efficiency (E%)	R²
18S	18S ribosomal RNA	TCTGGTCCTGTTCCGTTGG	124	100.21	0.983
18S	18S ribosomal RNA	GCTTTCGCAGTGGTTCGTC	124	100.21	0.983
ACT	actin	GTTGCCATTCAAGCCGTTCT	228	98.54	0.984
ACT	actin	AACAATTTCACGCTCAGCAGTAG	228	98.54	0.984
CYP	cyclophilin	TCTCGGGCAGCATTTCACGC	79	105.98	0.990
CYP	cyclophilin	AGCCGAAACTGGCGCCAACA	79	105.98	0.990
HIS4	histone H4	TTCCAGTTGGAAGGAAAAATGTCTG	253	107.83	0.981
HIS4	histone H4	GGCGAGCGTGCTCAGTGTAT	253	107.83	0.981
HSP70	heat shock protein 70	AACGCAAGGGCTTTGAGAA	139	103.40	0.980
HSP70	heat shock protein 70	ACCTGGCACGGGTTATGGT	139	103.40	0.980
PBL	serine/threonine-protein kinase	AGTTCTGCCATGGCCCGTGA	223	109.08	0.994
PBL	serine/threonine-protein kinase	TGCAGTGCCAACAACCGCTG	223	109.08	0.994
PGK1	phosphoglycerate kinase 1	GCGGGCGAGTAAAGTGGTA	181	95.14	0.990
PGK1	phosphoglycerate kinase 1	GGAGATCAAATACTTAATGGTGGGT	181	95.14	0.990
PP2A	protein phosphatase 2A	TGAAGGAGGGAGATTTGATTGA	128	107.83	0.988
PP2A	protein phosphatase 2A	CAGTTCCGATGCACTTGGGT	128	107.83	0.988
rbcl	large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase	CGTATTACAGTTCGGTGGAGGG	185	101.39	0.997
rbcl		CACAAGCGGCAGCTAGTTCA	185	101.39	0.997
RPL2	60S ribosomal protein L2	CCAGCATCGTTGTGGGAAAG	70	102.28	0.980
RPL2	60S ribosomal protein L2	GTGACCTCCTCCTCTATGTCGTAT	70	102.28	0.980
TUA2	tubulin α-2	CTTTCCTCGCACTCGCTGTT	181	109.94	0.992
TUA2	tubulin α-2	GGTGTAGGTAGGGCGGTCAA	181	109.94	0.992
UBC	ubiquitin-conjugating enzyme	CTCGCAGAATCATAAAGGAAACAC	180	107.09	0.981
UBC	ubiquitin-conjugating enzyme	CCATTGGATACTCTTCAGGCAAA	180	107.09	0.981

R², correlation coefficient.