Table 3.

Expression stability values of candidate reference genes calculated by BestKeeper.

Sample		1	2	3	4	5	6	7	8	9	10	11	12
Cold	gene	CYP	UBC	actin	rbcl	18S	PBL	PP2A	RPL2	PGK1	HSP70	HIS4	TUA2
	SD	0.35	0.38	0.44	0.46	0.47	0.70	0.71	0.74	0.76	0.79	1.00	1.31
	CV	1.29	1.69	1.97	2.94	7.37	2.73	2.79	3.87	3.47	3.46	3.76	5.75
Heat	gene	PP2A	CYP	18S	HSP70	PBL	UBC	HIS4	actin	rbcl	RPL2	PGK1	TUA2
	SD	0.42	0.66	0.67	0.68	0.78	0.78	0.80	0.92	1.04	1.04	1.12	1.19
	CV	1.62	2.36	10.67	2.91	2.98	3.49	2.97	4.08	5.52	4.68	4.78	4.99
Drought	gene	actin	18S	UBC	CYP	HSP70	TUA2	RPL2	PP2A	rbcl	PGK1	PBL	HIS4
	SD	0.33	0.35	0.35	0.42	0.53	0.58	0.66	0.69	0.74	0.82	0.88	1.05
	CV	1.52	5.60	1.61	1.57	2.33	2.60	3.31	2.75	4.79	3.81	3.46	4.22
Salt	gene	18S	rbcl	actin	UBC	CYP	HSP70	TUA2	PBL	PP2A	HIS4	RPL2	PGK1
	SD	0.38	0.39	0.40	0.45	0.57	0.60	0.68	0.72	0.78	0.84	0.96	1.12
	CV	6.18	2.45	1.78	2.03	2.11	2.65	3.08	2.85	3.06	3.28	4.88	5.15
ABA	gene	rbcl	PP2A	UBC	18S	CYP	actin	PBL	RPL2	PGK1	HSP70	TUA2	HIS4
	SD	0.37	0.47	0.56	0.66	0.72	0.74	0.87	0.87	0.92	0.96	1.05	1.15
	CV	2.26	1.81	2.51	9.82	2.64	3.27	3.32	4.28	4.11	4.12	4.50	4.43
GA3	gene	18S	CYP	rbcl	RPL2	UBC	actin	PBL	PP2A	HIS4	HSP70	TUA2	PGK1
	SD	0.46	0.47	0.47	0.53	0.59	0.65	0.66	0.69	0.78	0.78	0.79	0.96
	CV	7.20	1.76	2.88	2.67	2.71	2.95	2.56	2.74	3.00	3.47	3.51	4.41
MeJA	gene	UBC	18S	rbcl	PP2A	CYP	PGK1	actin	PBL	TUA2	RPL2	HIS4	HSP70
	SD	0.35	0.36	0.45	0.61	0.63	0.65	0.69	0.70	0.92	0.95	0.95	1.09
	CV	1.59	5.73	2.72	2.41	2.33	2.88	3.12	2.75	4.11	4.69	3.80	4.90
SA	gene	UBC	rbcl	CYP	actin	RPL2	HSP70	PBL	18S	HIS4	PGK1	TUA2	PP2A
	SD	0.35	0.35	0.41	0.44	0.54	0.64	0.82	0.85	0.87	0.91	0.98	1.05
	CV	1.54	2.19	1.52	1.96	2.74	2.82	3.24	14.33	3.43	4.09	4.33	4.20
Tissue	gene	18S	UBC	actin	CYP	PBL	PGK1	HIS4	HSP70	RPL2	PP2A	rbcl	TUA2
	SD	0.68	0.70	0.92	0.95	0.97	1.10	1.11	1.22	1.22	1.25	1.50	2.39
	CV	11.98	3.34	4.39	3.69	3.94	5.15	4.58	5.75	5.92	5.06	8.84	10.33
Abiotic	gene	18S	UBC	actin	CYP	PP2A	HSP70	PBL	PGK1	TUA2	HIS4	rbcl	RPL2
	SD	0.47	0.54	0.61	0.66	0.70	0.75	0.83	1.02	1.12	1.23	1.24	1.26
	CV	7.50	2.45	2.73	2.42	2.74	3.29	3.22	4.60	4.92	4.73	7.56	6.24
Hormone	gene	rbcl	UBC	18S	CYP	actin	RPL2	PBL	PP2A	PGK1	HSP70	HIS4	TUA2
	SD	0.50	0.56	0.60	0.62	0.67	0.75	0.78	0.82	0.88	0.91	0.96	1.03
	CV	3.05	2.51	9.51	2.29	3.03	3.73	3.03	3.21	3.98	4.01	3.74	4.52
Total	gene	18S	UBC	actin	CYP	PP2A	PBL	HSP70	PGK1	rbcl	RPL2	HIS4	TUA2
	SD	0.51	0.61	0.67	0.71	0.74	0.80	0.86	0.87	0.92	0.97	1.08	1.13
	CV	8.10	2.77	3.03	2.64	2.92	3.15	3.81	3.94	5.60	4.78	4.20	4.95