Table 5.
Significance of the main factors and their interactions on content of leaf free amino acids of MicroTom plants. Experimental design is as in Table 1.
| Alanine | Asparagine | GABA | Glutamine | Glutamate | Glycine | Proline | Serine | Threonine | Minor AA | Total AA | Gln:Glu | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
µmol g−1 FW |
||
| Nutrient solution (N) | * | *** | *** | *** | * | *** | *** | *** | *** | *** | *** | *** |
| 100% BNS | 1.01 a | 0.88 a | 2.39 a | 24.64 a | 1.94 a | 0.39 a | 0.58 a | 0.71 a | 0.34 a | 4.82 a | 37.47 a | 13.38 a |
| 70% BNS | 0.91 b | 0.76 b | 1.88 b | 11.74 b | 1.76 b | 0.30 b | 0.43 b | 0.57 b | 0.27 b | 3.90 b | 21.99 b | 6.93 b |
| Biostimulant (B) | *** | ns | *** | ns | ** | *** | *** | *** | *** | *** | *** | ns |
| Control | 0.64 b | 0.85 | 2.30 b | 17.57 | 1.76 b | 0.17 b | 0.39 b | 0.43 b | 0.19 b | 3.20 b | 26.82 b | 9.97 |
| Rygex | 0.61 b | 0.78 | 1.42 c | 17.90 | 1.76 b | 0.20 a | 0.57 a | 0.46 b | 0.19 b | 2.52 c | 26.67 b | 11.24 |
| Superfifty | 1.65 a | 0.83 | 2.70 a | 19.35 | 2.06 a | 0.68 a | 0.55 a | 1.04 a | 0.54 a | 7.37 a | 36.16 a | 9.33 |
| Salinity (S) | * | ns | ns | *** | * | ns | *** | ns | * | ns | *** | *** |
| S0 | 1.02 a | 0.86 | 2.14 | 15.11 b | 1.99 a | 0.36 | 0.32 a | 0.64 | 0.33 a | 4.46 | 26.76 b | 7.44 c |
| S1 | 0.89 b | 0.79 | 1.96 | 16.39 b | 1.79 b | 0.32 | 0.50 b | 0.61 | 0.28 b | 4.08 | 27.38 b | 9.43 b |
| S2 | 0.97 a | 0.81 | 2.30 | 23.22 a | 1.78 b | 0.35 | 0.69 c | 0.67 | 0.30 b | 4.54 | 35.22 a | 13.67 a |
| NxB | *** | *** | * | ** | *** | *** | ns | * | ns | *** | ** | *** |
| NxS | ns | ns | ns | ns | ns | * | *** | ns | ns | ns | ns | ns |
| BxS | * | *** | ** | *** | *** | ** | ns | * | * | *** | *** | ** |
| NxBxS | * | *** | * | ** | * | ns | *** | ns | ns | * | * | * |
| 100% Control S0 | 0.94 b | 1.04 abc | 2.67 abcde | 20.03 cdef | 2.22 ab | 0.32 | 0.28 fg | 0.65 | 0.31 | 4.12 d | 32.05 cde | 9.21 bcd |
| 100% Control S1 | 0.63 bc | 1.02 abcd | 2.70 abcd | 12.91 efg | 1.87 bc | 0.19 | 0.57 cd | 0.47 | 0.20 | 3.36 def | 22.77 efgh | 7.01 cd |
| 100% Control S2 | 0.79 bc | 1.10 a | 2.84 abc | 32.16 a | 2.02 abc | 0.15 | 0.57 cd | 0.50 | 0.17 | 3.95 de | 43.93 ab | 16.18 b |
| 100% Rygex S0 | 0.54 c | 0.69 efg | 1.26 f | 13.59 efg | 1.56 bcd | 0.16 | 0.30 efg | 0.43 | 0.20 | 2.30 fg | 21.67 efgh | 8.93 bcd |
| 100% Rygex S1 | 0.59 c | 0.72 defg | 1.47 cdef | 26.99 abc | 1.67 bcd | 0.20 | 0.69 bc | 0.49 | 0.19 | 2.50 fg | 36.22 bcd | 16.07 b |
| 100% Rygex S2 | 0.61 c | 0.74 cdef | 1.65 bcdef | 33.13 a | 1.44 cd | 0.23 | 0.91 ab | 0.51 | 0.20 | 2.84 efg | 42.99 ab | 24.95 a |
| 100% Superfifty S0 | 1.71 a | 1.08 ab | 3.40 a | 31.29 ab | 2.67 a | 0.79 | 0.29 gf | 1.12 | 0.64 | 8.09 ab | 50.23 a | 11.95 bcd |
| 100% Superfifty S1 | 1.51 a | 0.75 cdef | 2.73 abcd | 24.13 abcd | 1.92 abc | 0.74 | 0.57 cd | 1.06 | 0.57 | 8.20 a | 41.74 abc | 13.09 bc |
| 100% Superfifty S2 | 1.74 a | 0.75 cdef | 2.82 abc | 27.49 abc | 2.11 abc | 0.71 | 1.03 a | 1.13 | 0.55 | 8.02 ab | 45.59 ab | 13.06 bc |
| 70% Control S0 | 0.52 c | 0.94 abcde | 1.50 cdef | 11.61 fg | 1.81 bc | 0.11 | 0.18 g | 0.35 | 0.18 | 2.50 fg | 19.18 fgh | 6.56 cd |
| 70% Control S1 | 0.48 c | 0.42 g | 1.13 f | 6.81 g | 1.02 d | 0.08 | 0.29 efg | 0.20 | 0.08 | 2.02 g | 12.10 h | 6.99 cd |
| 70% Control S2 | 0.51 c | 0.60 fg | 2.98 ab | 21.91 bcde | 1.60 bcd | 0.15 | 0.48 cdef | 0.43 | 0.19 | 3.25 def | 30.89 cde | 13.88 bc |
| 70% Rygex S0 | 0.59 c | 0.61 fg | 1.37 def | 6.14 g | 1.70 bcd | 0.16 | 0.51 cdef | 0.36 | 0.16 | 1.98 g | 13.35 gh | 3.90 d |
| 70% Rygex S1 | 0.64 bc | 1.01 abcd | 1.50 cdef | 12.95 efg | 2.28 ab | 0.21 | 0.39 defg | 0.51 | 0.21 | 2.84 efg | 22.33 efgh | 5.93 cd |
| 70% Rygex S2 | 0.67 bc | 0.90 abcdef | 1.31 ef | 14.61 defg | 1.90 bc | 0.22 | 0.63 cd | 0.46 | 0.16 | 2.64 fg | 23.46 efg | 7.69 cd |
| 70% Superfifty S0 | 1.80 a | 0.78 bcdef | 2.65 abcde | 8.02 g | 1.99 abc | 0.62 | 0.38 defg | 0.93 | 0.50 | 7.80 ab | 24.05 efg | 4.09 d |
| 70% Superfifty S1 | 1.66 a | 0.84 abcdef | 2.31 abcdef | 14.11 efg | 2.03 abc | 0.58 | 0.45 cdef | 1.00 | 0.50 | 5.55 bc | 29.55 def | 7.05 cd |
| 70% Superfifty S2 | 1.48 a | 0.75 cdef | 2.22 abcdef | 10.03 g | 1.61 bcd | 0.62 | 0.55 cde | 0.99 | 0.49 | 6.56 c | 24.45 ef | 6.29 cd |
ns, *, **, *** Nonsignificant or significant at p ≤ 0.05, 0.01, and 0.001, respectively. Different letters indicate statistically different groups (p < 0.05, Tukey’s post hoc test following ANOVA; n = 3).