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. 2021 May 21;11(6):1491. doi: 10.3390/ani11061491

Figure 1.

Figure 1

Sperm tracks after CASA capture and track 3D reconstruction. (A) One field of rainbow trout (freshwater) sperm captured using SCA software (Microptic S.L.). The sperm swim in a tight helix. Red tracks = rapid progressive; green tracks = rapid non-progressive; blue tracks = slow non-progressive. (B) Detail of one sperm track reconstructed from X and Y coordinates every 100th of a second. (C) Three-dimensional reconstruction of track in B using the method of van der Horst and Sanchez [16]. (D) One field of mullet (seawater) sperm captured using SCA software (Microptic S.L.). Most sperm swim progressively forward. (E) Detail of one track reconstructed from X and Y coordinates every 100th of a second. Red tracks = rapid progressive; green track = rapid non-progressive; blue tracks = slow non-progressive. (F) Three-dimensional reconstruction of track in B using the method of van der Horst and Sanchez [16]. VCL = curvilinear velocity; ALH = amplitude of lateral head displacement; BCF = beat cross frequency.