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. 2021 May 21;11(6):1491. doi: 10.3390/ani11061491

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© 2021 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Examination of black-footed ferret (BFF) during electrostimulation. (B) Part of recording of sperm motility of the nine founder males. Detailed video recordings performed of sperm in cryopreservative medium before cryopreservation. (C) Sperm tracks of BFF sperm in TEST medium and (D) in equine extender causing hyperactivation and typical hyperactivated tracks. (E) Scanning electron micrograph of three normal Siberian ferret sperm (two still immature and retaining a cytoplasmic droplet). (F) BFF sperm showing multiple abnormalities, such as lipped acrosome and midpiece with Dag-defect. In BFF (Black footed ferret) and Siberian ferret, the posterior part of the acrosome is scallop-shaped.