Table 2.
The effect of mycotoxins AFB1 and OTA and the multicomponent mycotoxin detoxifying agent (MMDA) on the serum biochemical parameters in broiler chicks ( ± Std).
| Parameters (Unit) | Group A Negative Control |
Group B Mycotoxins |
Group C Mycotoxins+ MMDA 1 g/kg Feed |
Group D Mycotoxins+ MMDA 3 g/kg Feed |
|---|---|---|---|---|
| First Blood Sampling (25th day) | ||||
| Total Proteins * (g/dL) | 2.48 ± 0.26 | 2.39 ± 0.28 | 2.23 ± 0.39 | 2.34 ± 0.15 |
| Albumins * (g/dL) | 1.14 ± 0.14 | 1.06 ± 0.14 | 1.02 ± 0.13 | 1.01 ± 0.06 |
| ALP * (U/L) | 3932 ± 788 | 4613 ± 1561 | 5682 ± 1764 | 4887 ± 1486 |
| ALT * (U/L) | 9.69 ± 1.89 | 9.81 ± 4.64 | 7.75 ± 2.05 | 6.19 ± 1.52 |
| AST (U/L) | 111 ± 33 c | 176 ± 23 a | 168 ± 27 a | 125 ± 13 b |
| γ-GT * (U/L) | 5.75 ± 1.57 | 5.75 ± 1.65 | 5.88 ± 1.36 | 6.63 ± 1.26 |
| Glucose * (mg/dL) | 226 ± 22 | 213 ± 27 | 219 ± 14 | 228 ± 9 |
| Cholesterol (mg/dL) | 139 ± 15 a# | 129 ± 17 a | 127 ± 16 a# | 138 ± 17 a# |
| Second Blood Sampling (42nd day) | ||||
| Total Proteins * (g/dL) | 2.96 ± 0.69 | 2.64 ± 0.71 | 2.86 ± 0.87 | 2.69 ± 0.26 |
| Albumins * (g/dL) | 1.40 ± 0.30 | 1.25 ± 0.23 | 1.31 ± 0.34 | 1.24 ± 0.19 |
| ALP * (U/L) | 1727 ± 568 | 2005 ± 645 | 1796 ± 628 | 2012 ± 418 |
| ALT * (U/L) | 5.19 ± 1.76 | 5.8 ± 1.61 | 5.63 ± 2 | 5.25 ± 1.98 |
| AST (U/L) | 126 ± 14 a | 230 ± 39 b | 216 ± 39 b | 226 ± 22 b |
| γ-GT * (U/L) | 7.56 ± 2.16 | 8.27 ± 2.4 | 7.75 ± 2.65 | 7.81 ± 2.48 |
| Glucose * (mg/dL) | 202 ± 24 | 208 ± 31 | 214 ± 29 | 220 ± 20 |
| Cholesterol (mg/dL) | 112 ± 18 ab | 113 ± 14 ab | 121 ± 16 b | 107 ± 16 a |
# Means with symbol superscript in the same row suggest trend towards statistical significance (0.05 < p < 0.1). * Comparison of mean values of Albumins, glucose, ALP, ALT γ-GT are presented without superscripts since differences were observed without a Treatment × Time interaction in the statistical evaluation. p values Total Proteins at both blood samplings (Absence of Treatment × Time interaction observed): Group A vs. Group B = 0.096; A vs. C = 0.068. p values Albumins at both blood samplings (Absence of Treatment × Time interaction observed): Group A vs. Group B = 0.027; A vs. C = 0.022; A vs. D = 0.005. p values Glucose at both blood samplings (Absence of Treatment × Time interaction observed): Group B vs. Group D = 0.023. p values ALP at both blood samplings (Absence of Treatment × Time interaction observed): Group A vs. Group C = 0.082; A vs. D = 0.047. p values ALT at both blood samplings (Absence of Treatment × Time interaction observed): Group A vs. Group D = 0.021; B vs. D = 0.007. a,b,c Means with different superscripts in the same row differ significantly (p < 0.05) and Treatment × Time interaction at the specific parameter statistical evaluation was observed. p values Cholesterol at First blood sampling (Treatment × Time interaction observed): Group A vs. Group C = 0.072; C vs. D = 0.090; at 2nd blood sampling (Treatment × Time interaction observed): Group C vs. Group D = 0.012. p values AST at First blood sampling (Treatment × Time interaction observed): All comparisons among groups with different superscripts < 0.001, except Group A vs. Group D = 0.007; at 2nd blood sampling (Treatment × Time interaction observed): Group A vs. all groups < 0.001.