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Primer sequences used for real-time quantitative PCR.
| Genes | Primer Sequence (5′–3′) | |
|---|---|---|
| UCP1 | Forward | ACTGCCACACCTCCAGTCATT |
| Reverse | CTTTGCCTCACTCAGGATTGG | |
| SOAT1 | Forward | AACTCCATCTTGCCAGGTGTCTTG |
| Reverse | ACCACGTTCCAGGTCCTGTAGTAG | |
| ACC | Forward | TTGAAGGCACAGTGAAGGCTTACG |
| Reverse | GACGCCATCTTCCTCTGTCAGTTG | |
| CPT1A | Forward | GTGGCATCTCCTTTAACTCAAC |
| Reverse | CGGCGTTGAAGATCTTGTATTC | |
| β-actin | Forward | GACATTTGAGAAGGGCCACAT |
| Reverse | CAAAGAGGTCCAAAACAATCG | |
