Table 4.
Description of tested methods.
| Designation | Primers (5′-3′) | Probe (5′-3′) | Target | Amplicon Size (bp) | Thermocycling Conditions | Total Duration |
|---|---|---|---|---|---|---|
| Fontaine et al. 2002 [13] method | F:CGCTTCTCTAGCCTTTCATGA R: CTTCACGTGTGTTTGCCAAT |
CCAATCACAGAATCATCAGAATCGACTGGTATC | Specific C. parvum sequence |
138 | 50 °C—2 min 95 °C—10 min 40 cycles: 95 °C—15 s/60 °C—1 min |
62 min |
| Valeix et al. 2020 [22] method | F: GTTAAACTGCRAATGGCT R: CGTCATTGCCACGGTA |
CCGTCTAAAGCTGATAGGTCAGAAACTTGAATG and GTCACATTAATTGTGATCCGTAAAG | 18S rRNA | 258 | 95 °C—10 min 50 cycles: 95 °C—15 s/50 °C—15 s (touchdown from 60 °C)/72 °C—15 s |
48 min |
| Hadfield et al. 2011 [14] method | F:GAGGTAGTGACAAGAAATAACAATACAGG R:CTGCTTTAAGCACTCTAATTTTCTCAAAG |
TACGAGCTTTTTAACTGCAACAA | 18S SSU rRNA | 300 | 95 °C—10 min 55 cycles: 95 °C—15 s/60 °C—60 s |
78 min |
| Mary et al. 2013 [15] method | F: CATGGATAACCGTGGTAAT R: TACCCTACCGTCTAAAGCTG |
CTAGAGCTAATACATGCGAAAAAA | 18S rRNA | 178 | 94 °C—10 min 45 cycles: 94 °C—10 s/54 °C—30 s/72 °C—10 s |
48 min |
| RIDA® GENE Parasitic Stool Panel II | Not disclosed | Not disclosed | Not disclosed | Not disclosed | 95 °C—1 min 45 cycles: 95 °C—15 s/60 °C—30 s |
35 min |
| FTD® Stool parasites | Not disclosed | Not disclosed | DNA J-like protein gene | Not disclosed | 50 °C—15 min 94 °C—1 min 40 cycles: 94 °C—8 s/60 °C—1 min |
62 min |
| Amplidiag® Stool Parasites | Not disclosed | Not disclosed | COWP gene | Not disclosed | 95 °C—10 min 45 cycles: 95 °C—15 s/65 °C—1 min |
66 min |
| Allplex® GI Parasite Assay | Not disclosed | Not disclosed | Not disclosed | Not disclosed | 50 °C—20 min 95 °C—15 min 45 cycles: 95 °C—10 s/60 °C—1 min/72 °C—30 s |
110 min |
F: Forward. R: Reverse. SSU: Small subunit. COWP: Cryptosporidium oocyst wall protein.