Table 4.
A computer-aided sperm analysis of the kinematic parameters of frozen-thawed bull sperm after a combined AP and APS addition (mmol/L + mg/mL).
| 0.2 AP | 0.2 AP + 0.1 APS | 0.2 AP + 0.3 APS | 0.2 AP + 0.5 APS | 0.2 AP + 0.7 APS | |
|---|---|---|---|---|---|
| TM (%) | 39.75 ± 1.15 b | 42.54 ± 1.42 a | 43.21 ± 1.41 a | 41.79 ± 1.10 ab | 39.52 ± 1.16 b |
| DAP (μm) | 10.31 ± 0.99 c | 12.55 ± 0.84 ab | 12.91 ± 0.98 a | 12.10 ± 1.16 ab | 10.88 ± 0.83 bc |
| DSL (μm) | 10.15 ± 1.11 b | 12.22 ± 0.99 a | 12.79 ± 1.11 a | 11.14 ± 0.96 ab | 11.00 ± 1.05 ab |
| DCL (μm) | 22.89 ± 1.67 | 22.15 ± 1.46 | 20.85 ± 1.85 | 22.45 ± 1.56 | 21.07 ± 2.18 |
| VAP (μm/s) | 33.50 ± 1.63 b | 35.97 ± 1.21 a | 36.75 ± 0.97 a | 34.91 ± 0.91 ab | 33.64 ± 1.18 b |
| VSL (μm/s) | 26.71 ± 0.78 b | 28.14 ± 1.15 ab | 29.26 ± 1.18 a | 27.24 ± 0.93 ab | 26.48 ± 2.01 b |
| VCL (μm/s) | 66.99 ± 2.83 | 66.59 ± 2.38 | 65.30 ± 2.35 | 66.45 ± 2.37 | 65.69 ± 2.60 |
| STR(VSL/VAP) (%) | 80.02 ± 5.89 | 78.31 ± 3.91 | 79.64 ± 3.69 | 78.12 ± 4.00 | 78.92 ± 7.78 |
| LIN (VSL/VCL) (%) | 39.97 ± 2.45 | 42.34 ± 2.74 | 44.82 ± 1.55 | 41.03 ± 1.52 | 40.47 ± 4.53 |
| WOB(VAP/VCL) (%) | 50.01 ± 1.37 c | 54.04 ± 1.45 ab | 56.32 ± 1.10 a | 52.58 ± 1.94 bc | 51.30 ± 2.94 bc |
| ALH (μm) | 3.46 ± 0.27 | 3.34 ± 0.13 | 3.46 ± 0.28 | 3.39 ± 0.20 | 3.26 ± 0.11 |
| BCF(hz) | 10.35 ± 1.12 | 10.69 ± 0.91 | 10.68 ± 0.99 | 10.62 ± 0.93 | 10.29 ± 1.26 |
Abbreviations: Mean ± SEM of the total motility (TM), average path distance (DAP), straight line distance (DSL), curve distance (DCL), average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR), linearity (LIN), wobble (WOB), amplitude of lateral head displacement (ALH), and beat/cross frequency (BCF). The different superscripted letters (a, b, c) across the rows represent significant differences (p < 0.05).