Table 2.
Mass spectrometric analysis of the proteins of INS-1 cells persistently infected with CV-B4.
| Accession | Protein | Infected Cells Score | Controls Score | Score Difference | p-Value |
|---|---|---|---|---|---|
| P21807 | Peripherin | 0.00 ± 0.00 | 12.56 ± 4.10 | −12.56 | *** |
| Q07266-2 | Isoform E1 of Drebrin | 6.29 ± 3.00 | 17.56 ± 2.90 | −11.27 | *** |
| Q4QQS7 | Protein Umps | 0.49 ± 0.99 | 5.40 ± 1.14 | −4.91 | *** |
| P34926 | Microtubule-associated protein 1A | 2.00 ± 1.37 | 14.30 ± 5.17 | −12.3 | ** |
| Q5XIM9 | T-complex protein 1 subunit beta | 7.86 ± 3.07 | 15.90 ± 2.48 | −8.04 | ** |
| P23514 | Coatomer subunit beta | 1.05 ± 2.10 | 5.01 ± 1.13 | −3.96 | ** |
| D4A2G9 | Protein Ranbp1 | 0.00 ± 0.00 | 3.60 ± 1.79 | −3.6 | ** |
| G3V7N5 | Carnitine O-palmitoyltransferase 2, mitochondrial | 0.00 ± 0.00 | 3.38 ± 1.66 | −3.38 | ** |
| D3ZMY7 | Protein Nt5c2 | 0.43 ± 0.87 | 3.75 ± 1.12 | −3.32 | ** |
| F1LMC7 | Septin-7 | 0.44 ± 0.87 | 2.60 ± 1.37 | −2.16 | ** |
| A0A0G2K1J5 | Plectin | 4.04 ± 2.38 | 21.11 ± 13.47 | −17.07 | * |
| Q6URK4 | Heterogeneous nuclear ribonucleoprotein A3 (HNRPA3) | 0.00 ± 0.00 | 16.28 ± 13.66 | −16.28 | * |
| P12785 | Fatty acid synthase | 6.78 ± 3.89 | 22.06 ± 8.44 | −15.28 | * |
| A0A0G2JU82 | Microtubule-actin cross-linking factor 1 | 7.59 ± 5.23 | 22.14 ± 9.29 | −14.55 | * |
| Q05982 | Nucleoside diphosphate kinase A | 6.26 ± 8.23 | 20.65 ± 6.46 | −14.39 | * |
| Q6URK4-2 | Heterogeneous nuclear ribonucleoprotein A3 (HNRPA3) isoform 2 | 18.03 ± 4.53 | 4.98 ± 8.08 | +13.05 | * |
| A0A0G2K013 | Alpha-actinin-4 | 9.04 ± 1.59 | 21.08 ± 7.58 | −12.04 | * |
| P06687 | Sodium/potassium-transporting ATPase subunit alpha-3 | 0.00 ± 0.00 | 11.90 ± 7.78 | −11.9 | * |
| D4AD15 | Protein Eif4g1 | 4.66 ± 2.13 | 16.16 ± 6.89 | −11.5 | * |
| A0A0G2K0Q7 | Protein Mylk | 3.17 ± 2.13 | 11.74 ± 4.47 | −8.57 | * |
| O35314 | Secretogranin-1 | 5.04 ± 1.57 | 13.22 ± 4.74 | −8.18 | * |
| Q62667 | Major vault protein | 6.15 ± 2.75 | 14.06 ± 4.71 | −7.91 | * |
| D4AC23 | Protein Cct7 | 6.14 ± 1.77 | 13.98 ± 4.59 | −7.84 | * |
| P04692-5 | Tropomyosin alpha-1 chain isoform 5 | 1.62 ± 3.23 | 8.57 ± 5.17 | −6.95 | * |
| Q3MIE4 | Synaptic vesicle membrane protein VAT-1 homolog | 2.34 ± 2.20 | 9.00 ± 3.48 | −6.66 | * |
| D3ZRM9 | Uncharacterized protein | 0.00 ± 0.00 | 6.54 ± 3.76 | −6.54 | * |
| D3ZRM9 | Uncharacterized protein | 0.00 ± 0.00 | 6.54 ± 3.76 | −6.54 | * |
| Q62950 | Dihydropyrimidinase-related protein 1 | 2.41 ± 3.70 | 8.67 ± 4.13 | −6.26 | * |
| F1MAA1 | Ubiquitin-specific peptidase 47 | 2.26 ± 2.61 | 8.50 ± 4.06 | −6.24 | * |
| O35303-6 | Dynamin-1-like protein isoform 6 | 1.91 ± 3.83 | 7.53 ± 2.90 | −5.62 | * |
| P41562 | Isocitrate dehydrogenase [NADP] cytoplasmic | 2.57 ± 3.14 | 8.07 ± 2.98 | −5.5 | * |
| D4A0C3 | Protein Hid1 | 1.38 ± 1.78 | 6.44 ± 2.39 | −5.06 | * |
| A0A0G2JZ60 | Protein Fsd1l | 3.86 ± 3.10 | 8.89 ± 2.01 | −5.03 | * |
| A0A0G2JUN7 | Thioredoxin reductase 1, cytoplasmic | 2.77 ± 1.97 | 7.38 ± 2.33 | −4.61 | * |
| D3ZVQ0 | Protein LOC100911959 | 2.72 ± 2.66 | 7.14 ± 1.73 | −4.42 | * |
| O70593 | Small glutamine-rich tetratricopeptide repeat-containing protein alpha | 1.03 ± 1.21 | 5.43 ± 2.75 | −4.4 | * |
| P50475 | Alanine--tRNA ligase, cytoplasmic | 0.44 ± 0.88 | 4.76 ± 2.41 | −4.32 | * |
| P27008 | Poly [ADP-ribose] polymerase 1 | 1.90 ± 2.77 | 6.15 ± 2.62 | −4.25 | * |
| B2RYI2 | Signal recognition particle subunit SRP68 | 2.03 ± 1.60 | 6.04 ± 1.87 | −4.01 | * |
| Q05096-3 | Unconventional myosin-Ib isoform 3 | 1.96 ± 2.70 | 5.89 ± 1.41 | −3.93 | * |
| O88321 | Antisecretory factor | 1.47 ± 1.86 | 5.33 ± 2.30 | −3.86 | * |
| B2GV74 | Kinesin light chain 2 | 1.08 ± 2.16 | 4.89 ± 2.36 | −3.81 | * |
| Q9Z1W6-4 | Protein LYRIC isoform 4 | 0.54 ± 1.08 | 3.58 ± 2.25 | −3.04 | * |
| A0A0H2UHW4 | PEST proteolytic signal-containing nuclear protein | 0.42 ± 0.85 | 3.29 ± 2.02 | −2.87 | * |
| E9PT23 | Putative sodium-coupled neutral amino acid transporter 10 | 0.46 ± 0.93 | 3.00 ± 1.92 | −2.54 | * |
| D3Z8U5 | Metalloendopeptidase | 0.87 ± 1.73 | 3.10 ± 1.25 | −2.23 | * |
| Q5M7W6 | Protein FAM234A | 5.24 ± 0.98 | 1.88 ± 2.18 | +3.36 | * |
Mock and CV-B4-infected INS-1 β cells were lysed in a buffer containing SDS to extract the proteins. The proteins were analyzed using Shotgun mass spectrometry. The results are expressed as the SEQUEST protein identification score. The difference in score between infected cells and controls was calculated. Proteins from infected cells for which the identification score value is 0 are underlined. Results are presented as the mean ± standard deviation of six replicates. *: p < 0.05, **: p < 0.005, ***: p < 0.0005. In bold: score difference > 0. Unerlined: infected cells score = 0.