Skip to main content
. 2021 Jun 24;17(6):e1009636. doi: 10.1371/journal.ppat.1009636

Fig 5. EBOV VP24 inhibits Kα1-STAT3 interaction, dependent on STAT1.

Fig 5

(A) HEK293T or U3A cells co-transfected to express the indicated proteins were treated 24 h post-transfection with or without OSM (10 ng/ml, 15 min) before lysis and immunoprecipitation for FLAG. Lysates (input) and immunoprecipitates (IP) were analysed by IB using antibodies against the indicated proteins. Expanded images of all membranes are shown in S8 Fig. (B) Images of membranes such as those shown in (A) were analysed using Image Lab software to calculate the intensity of bands for pY-STAT1 and pY-STAT3 in IP samples; values for the different samples were calculated relative to the intensity of the corresponding FLAG-Kα1 IP band and then normalised to control samples (FLAG-Kα1/GFP treated with OSM); the histograms show mean ± SD, n ≥ 2 assays.