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. 2021 Jun 24;17(6):e1009636. doi: 10.1371/journal.ppat.1009636

Fig 7. EBOV VP24 interacts with STAT3.

Fig 7

(A) U3A cells co-transfected to express FLAG-STAT3 and GFP or GFP-VP24 as indicated were treated 24 h post-transfection with or without OSM (10 ng/ml, 30 min) before lysis, immunoprecipitation for GFP, and IB, as described in the legend to Fig 5. (B, C) U3A (B) or HEK293T (C) transfected to express the indicated proteins were treated with or without OSM (10 ng/ml, 15 min) before immunoprecipitation for GFP and IB for endogenous STAT3. Results are representative of 2 independent assays and show data from a single blot with intervening and marker lanes removed. Expanded images of all membranes are shown in S9 and S10 Figs.