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. 2021 Jun 24;17(6):e1009636. doi: 10.1371/journal.ppat.1009636

Fig 8. Antagonism of STAT3 by EBOV VP24 in U3A cells is independent of VP24-karyopherin interaction.

Fig 8

(A) upper panel: HEK293T or U3A cells co-transfected with pISRE-LUC or m67-LUC plasmid, pRL-TK plasmid, and plasmids to express the indicated proteins, were treated 8 h (IFN-α) or 16 h (OSM) post-transfection with or without IFN-α (1,000 U/ml for 16 hours) or OSM (10 ng/ml for 8 h) before determination of relative luciferase activity (mean ± SEM; n ≥ 3 independent assays); lower panel: cell lysates used in representative assays were analysed by IB for GFP and β-tubulin. Statistical analysis used Student’s t-test; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; NS, not significant. (B) U3A cells transfected to express the indicated proteins were treated with OSM before immunoprecipitation for GFP and IB, as described in the legend to Fig 7. Results are representative of 2 independent assays and show data from a single blot with intervening and marker lanes removed. Expanded images of membranes are shown in S11 Fig.