Table 2. Results of IS6110-targeted PCR (n = 81).
Participants | IS6110-target qPCR Ct values | ddPCR-estimated IS6110 copy/20ul | MTB-specific IFN-γ (IU/mL) |
---|---|---|---|
PTB cases | |||
1 | 31.43 | 136.0 | 6.43 |
2 | 31.77 | 612.0 | 8.12 |
3 | 32.02 | 138.0 | 9.90 |
4 | 32.31 | 416.0 | 9.94 |
5 | 33.57 | 20.0 | 1.46 |
6 | 35.64 | 62.0 | 6.99 |
7 | 36.01 | ND | 7.74 |
8 | 36.31 | ND | 9.93 |
9 | 37.23 | 2.8 | 9.95 |
10 | 37.25 | 0 | 6.17 |
11 | 37.85 | 1.4 | 4.79 |
12 | 37.93 | 0 | 9.19 |
13 | 38.20 | 1.4 | 3.58 |
14 | 38.58 | 0 | 0.84 |
15 | 38.73 | 0 | 0.48 |
16 | 39.80 | 0 | 0.10 |
17 | 44.83 | 0 | 0 |
18–24 | >45.00 | 0 | 4.50±3.65 |
LTBI controls | |||
1 | 33.64 | 104.0 | 7.60 |
2 | 36.93 | 0 | 3.14 |
3–57 | >45.00 | - | 2.69±2.88 |
Abbreviations: ddPCR, digital droplet polymerase chain reaction (PCR); LTBI, latent tuberculosis infection; ND, “not done” because of inadequate samples for ddPCR tests; MTB-specific IFN-γ, interferon-gamma released after Mycobacterium tuberculosis–specific antigen stimulation; PTB, pulmonary tuberculosis (TB); qPCR Ct value, quantitative PCR–derived cycle threshold value.