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. 2021 Jun 24;16(6):e0253879. doi: 10.1371/journal.pone.0253879

Table 2. Results of IS6110-targeted PCR (n = 81).

Participants IS6110-target qPCR Ct values ddPCR-estimated IS6110 copy/20ul MTB-specific IFN-γ (IU/mL)
PTB cases
    1 31.43 136.0 6.43
    2 31.77 612.0 8.12
    3 32.02 138.0 9.90
    4 32.31 416.0 9.94
    5 33.57 20.0 1.46
    6 35.64 62.0 6.99
    7 36.01 ND 7.74
    8 36.31 ND 9.93
    9 37.23 2.8 9.95
    10 37.25 0 6.17
    11 37.85 1.4 4.79
    12 37.93 0 9.19
    13 38.20 1.4 3.58
    14 38.58 0 0.84
    15 38.73 0 0.48
    16 39.80 0 0.10
    17 44.83 0 0
    18–24 >45.00 0 4.50±3.65
LTBI controls
    1 33.64 104.0 7.60
    2 36.93 0 3.14
    3–57 >45.00 - 2.69±2.88

Abbreviations: ddPCR, digital droplet polymerase chain reaction (PCR); LTBI, latent tuberculosis infection; ND, “not done” because of inadequate samples for ddPCR tests; MTB-specific IFN-γ, interferon-gamma released after Mycobacterium tuberculosis–specific antigen stimulation; PTB, pulmonary tuberculosis (TB); qPCR Ct value, quantitative PCR–derived cycle threshold value.