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(A) We genotyped 7 progeny with the Lemon Frost phenotype and 6 wild type progeny from the third generation of a backcross of Mr. Frosty to the Sunburst line for markers in the SPINT1 region and observed a consistent inheritance pattern. (B) Sequencing chromatogram of a heterozygous animal (lf/+) at an insertion marker. (C) Sequencing chromatogram of a homozygous animal (+/+) at the same insertion marker.
