KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Mtor | gift from Prof. K.M. Johansen (Iowa State University) and available at Developmental Studies Hybridoma Bank | Clone 12F10, RRID: AB_2721935 |
| Rabbit polyclonal anti-Lamin DmO | gift from Prof. P. Fisher (SUNY StonyBrook School of Medicine) | Clone L7 |
| Rabbit anti-MOF | Gift from Prof M.I. Kuroda (Harvard University) | N/A |
| Rabbit polyclonal anti-MLE | Gift from Prof M.I. Kuroda (Harvard University) | N/A, RRID: AB_2568421 |
| Rabbit polyclonal anti-H4K16ac | Santa Cruz Biotechnology, Inc. | Cat# sc-8662, RRID: AB_634804 |
| Rabbit polyclonal anti-Histone H3 | Abcam | Cat#ab1791, RRID:AB_302613 |
| Mouse monoclonal anti-HP1 | Developmental Studies Hybridoma Bank | Clone C1A9, RRID:AB_528276 |
| Mouse monoclonal anti-EcR | Developmental Studies Hybridoma Bank | Clone DDA2.7, RRID:AB_528209 |
| Chicken polyclonal anti-GFP | Aves Labs, Inc. | Cat#1020, RRID:AB_10000240 |
| Rabbit monoclonal anti-H3K27me3 | Cell Signaling Technology | Cat#9733, RRID:AB_2616029 |
| Rabbit polyclonal anti-H3K9me3 | Abcam | Cat#ab8898, RRID:AB_306848 |
| Goat anti-mouse IgG secondary Alexa Fluor 488 | Thermo Fisher Scientific | Cat # A32723, RRID:AB_2633275 |
| Goat anti-rabbit IgG secondary Alexa Fluor 488 | Thermo Fisher Scientific | Cat # A32731, RRID:AB_2633280 |
| Goat anti-mouse IgG secondary Alexa Fluor 568 | Thermo Fisher Scientific | Cat # A-11004, RRID:AB_2534072 |
| Goat anti-rabbit IgG secondary Alexa Fluor 568 | Thermo Fisher Scientific | Cat # A-11011, RRID:AB_143157 |
| Biological samples | ||
| Drosophila 3rd instar larval salivary glands | This study | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Dynabeads Protein A | Thermo Fisher Scientific | Cat#10002D |
| Power SYBR Green PCR Master mix | Applied Biosystems | Cat#4367659 |
| Sigmacote | Sigma Aldrich | Cat#SL2 |
| Poly L-lysinate slides | Polysciences | Cat#22247 |
| ProLong Gold Antifade | Thermo Fisher Scientific | Cat# P36930 |
| VECTASHIELD® Antifade Mounting Medium | Vector Laboratories | Cat#H-1000-10 |
| Hoechst 33342 | Thermo Fisher Scientific | Cat#H3570 |
| Hybridization Buffer | Biosearch Technologies | Cat# SMF-HB1-1 |
| Wash Buffer A | Biosearch Technologies | Cat# SMF-WA1-60 |
| Wash Buffer B | Biosearch Technologies | Cat# SMF-WB1-20 |
| Dynabeads® Oligo (dT)25 | Thermo Fisher Scientific | Cat#61002 |
| SPRIselect beads | Beckman Coulter | Cat# B23317 |
| EZ-link HPDP Biotin | Thermo Fisher Scientific | Cat#21341 |
| 4-thiouridine (4sU) | Sigma-Aldrich | Cat# T4509 |
| Dynabeads MyOne Streptavidin C1 | Thermo Fisher Scientific | Cat#65002 |
| Critical commercial assays | ||
| NEBNext Library Quant Kit for Illumina | NEB | E7630L |
| OneStep RT-PCR kit | QIAGEN | Cat#210212 |
| MEGAscript T7 Transcription kit | Thermo Fisher Scientific | AM1334 |
| Pure Link RNA mini kit | Thermo Fisher Scientific | 12183018A |
| NextSeq 500/550 High Output v2 kit (75 cycles) | Illumina | FC-404-2005 |
| Bioanalyzer High Sensitivity RNA Analysis Kit | Agilent | Cat#5067-1513 |
| Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | Cat#23225 |
| Deposited data | ||
| RNA-Seq 3rd instar larval salivary glands | This study | GEO: GSE155323 |
| TT-Seq S2 cells | This study | GEO: GSE155323 |
| Experimental models: Cell lines | ||
| S2-DRSC | Drosophila Genomics Resource Center | Flybase: FBrf0024118 |
| Experimental models: Organisms/strains | ||
| Mtor RNAi line - w[1118]; Mi{GFP[E.3×P3] = ET1}side-VIII[MB03736] | Bloomington Drosophila Stock Center | #24265 |
| MOF RNAi line - y[1] v[1]; P{y[+t7.7] v [+t1.8] = TRiP.JF01707}attP2 | Bloomington Drosophila Stock Center | #31401 |
| Nubbin-Gal4 line - w[*]; P{w[nub.PK] = nub-GAL4.K}2 | Bloomington Drosophila Stock Center | #86108 |
| mCD8-RFP line - w[*]; P{w[+mC] = UAS-mCD8.ChRFP}3 | Bloomington Drosophila Stock Center | #27392 |
| Mtor allele - y[1] w[67c23]; P{w[+mC] = lacW}Mtor[k03905]/CyO | Bloomington Drosophila Stock Center | #10537 |
| Heatshock clone line (Act5c, FRT y+ FRT, Gal4, UAS-GFP) - y[1] w[*]; P{w[+mC] = AyGAL4}25 P{w[+mC] = UAS-GFP.S65T} Myo31DF[T2] | Bloomington Drosophila Stock Center | #4411 |
| Heatshock clone line - y1, w*, hsFLP; Kr/CyO; MKRS/TM6B | Gift from Prof. S. DiNardo (University of Pennsylvania) | N/A |
| roX1/2 allele - roX1SMC17A roX2Δw+; +/ CyO[w+roX1] | Gift from Prof. V. Meller (Wayne State University) | N/A |
| Oregon-R | Bloomington Drosophila Stock Center | #5 |
| w118 - w[118];Df(2R)H3D3/CyO | Bloomington Drosophila Stock Center | #31 |
| Oligonucleotides | ||
| Expression analysis primers | IDT | See Table S1 |
| dsRNA primers | IDT | See Table S1 |
| RNA FISH probes | Biosearch Technologies | See Table S1 |
| Software and algorithms | ||
| Fiji | Schindelin et al., 2012 | https://fiji.sc/ |
| Leica Application Suite X | Leica software | https://www.leica-microsystems.com/products/microscope-software/software-for-life-science-research/las-x-powerful-and-flexible/ |
| Prism | GraphPad software | https://www.graphpad.com/scientific-software/prism/ |
| Biorender | Biorender software | https://biorender.com/ |
| R | The R Project for Statistical Computing | https://www.r-project.org/ |
| STAR (v2.3.0e) | Dobin et al., 2013 | https://github.com/alexdobin/STAR |
| SAMtools (v1.1) | Li et al., 2009 | http://samtools.sourceforge.net/ |
| RSeQC (v3.0.1) | Wang et al., 2012 | http://rseqc.sourceforge.net/ |
| featureCounts (v1.6.2) | Liao et al., 2014 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| DESeq2 (v1.30.1) | Love et al., 2014 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| BEDtools (v2.27.1) | Quinlan and Hall, 2010 | https://bedtools.readthedocs.io/en/latest/ |
| Deeptools (v2.5.7) | Ramírez et al., 2016 | https://github.com/deeptools/deepTools |
| Python | Python Software Foundation | https://www.python.org |
| MATLAB | MATLAB software | https://www.mathworks.com/products/matlab.html |