Table 1.

Masses of species detected with SEC-nMS. Relative quantification of the different species was assessed based on SEC-UV signals. * ND = mass not determined because of very low intensities on the nMS spectrum.

	T0	T1	T2			T-GlyCLICK-DM1
Main Product	99.6%	99.7%	96.6%			98.5%
	G0F/G0 147,930 ± 4 Da	145,875 ± 1 Da	146,372 ± 2 Da			148,957 ± 1 Da
	(G0F)2 148,067 ± 4 Da
	G1F/G0F 148,228 ± 2 Da
	(G1F)2 148,387 ± 2 Da
	G2F/G1F 148,548 ± 1 Da
HMW Dimers	0.4%	0.3%	0.5%			0.2%
	296,828 ± 25 Da	291,719 ± 25 Da	292,911 ± 23 Da			ND *
LMWS	-	-	2.7%	Fc-Fab	99,319 ± 6 Da	1.3%	Fc-Fab	101,910 ± 8 Da
			0.2%	LC	23,473 ± 2 Da	<0.1%	LC	23,474 ± 3 Da
				Fd	23,618 ± 4 Da		Fd	23,615 ± 3 Da
				Fab	47,129 ± 9 Da		Fab	47,091 ± 8 Da