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. 2021 May 24;12(6):798. doi: 10.3390/genes12060798

Figure 1.

Figure 1

(A) Pedigree of the studied family with CGG sizing determined using an AmplideX TP-PCR. Males are represented by squares, females by circles and triangle sex unknown. A black outline indicates examined and half shaded indicates carrier. A black arrow indicates proband. III:1 Miscarriage at 6 of 40 weeks; (B) AmplideX TP-PCR CGG sizing, with no FM alleles detected in either III:3 male CVS or II:2 proband; (C) II:2 and (D) III:3 AmplideX mPCR, with the upper panel in blue representing CGG sizing based on control digestions and the lower panel in green representing relative methylation based on HpaII methylation sensitive restriction enzyme-based digestion followed by long-range PCR. No FM alleles are detected in either III:3 male CVS or II:2 female. Skewed methylation for the 75 CGG PM allele is suggested by 80% methylation. Note: numbers on each panel next to specific peaks indicate CGG sizes.