Figure 2.

(A) Pedigree of the studied family with CGG sizing determined using Southern blot and standard CGG sizing PCR. Males are represented by squares, females by circles and triangle sex unknown. The black outline indicates examined, half shaded indicates carrier, and full shaded indicates affected with FXS. The black arrows indicate proband and a diagonal line indicates being deceased. III:1 Miscarriage at 6 of 40 weeks (B) Southern blot Lane 1 (male PM control), Lane 2 (III:3 male PM/FM CVS), Lane 3 (II:2 PM/FM female), Lane 4 (male normal CGG size control).