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(a) One representative Western blot analysis demonstrating the reduction in growth factors and β-catenin expression in Onco-P20-treated (0.15 μg/mL) NHFs. Proteins were extracted after 2 weeks of treatment. (b) Immuno-enzymatic quantification of IGFBP4, IGFBP6, and VEGF released in the cell culture medium. After 2 weeks of treatment, cells were incubated for an additional 48 h with DMEM without FBS before medium collection. Results were normalized against the protein concentration.
