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. 2021 May 24;9(6):117. doi: 10.3390/toxics9060117

Figure 5.

Figure 5

Figure 5

Effect of AChR inhibitor and Ca-chelating agent on isolated mouse phrenic nerve–diaphragm. (a) Effects of AChR inhibitor (NTX) on isolated mouse phrenic nerve–diaphragm. The organ baths were pretreated with NTX (a nicotinic AChR inhibitor) for 15 min. Thereafter, 8 mM ACR was added after nerve- or muscle-evoked twitches were observed, and the mouse phrenic nerve–diaphragm was incubated for 80 min. After 80 min of ACR treatment, samples of mouse phrenic nerve–diaphragm were refreshed with physiological buffer solution. Control: only NTX treatment; NTX and ACR treatment. (b) Effects of calcium-chelating agent on isolated mouse phrenic nerve–diaphragm. The phrenic nerve–diaphragm baths were incubated with either Krebs solution or a Ca2+-free (with 50 µM EGTA) modified Krebs solution. Thereafter, 8 mM ACR was added after the tension returned to the baseline level. Control: only EGTA treatment; EGTA and ACR treatment.