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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Adv Ther (Weinh). 2021 Apr 23;4(6):2100036. doi: 10.1002/adtp.202100036

Figure 4. NP-SOD administration minimizes acute oxidative injury following I/R in vivo.

Figure 4.

A-B) Explanted hearts were flushed with PBS and cut into 2mm sections along the short axis. Apical, mid, and basilar sections were incubated in 1% 2,3,5-Triphenyltetrazolium chloride (TTC) for 20 minutes at 37°C before fixation in 4% PFA. Sections were imaged and the area of ischemic tissue across all apical and middle regions was quantified as a portion of the whole myocardium demonstrating the myocardial area at risk. C) LV myocardium was lysed and precipitated with Thiobarbituric Acid (TBA). MDA concentration was determined by colorimetric detection of TBA-MDA adduct formation as a measure of ROS induced lipid peroxidation. D) At 3-hours post I/R, echocardiography was performed across short and long axes. End-systolic and end-diastolic volumes were obtained in order to derive LV ejection fraction. Treatment groups assessed were as follows: Sham (n=4), PBS (n=6), free SOD (n=5), NP-SOD (n=7). Significance represented as follows: *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.