Figure 5. Chronic ventricular remodeling following I/R injury is attenuated by NP-SOD treatment.
Hearts were explanted at 28-days post I/R, washed, sectioned and mounted at 10 μM in OCT. Sections including mid-papillary muscles in the LV were selected as representative regions affected by previous I/R injury. Masson’s Trichrome and Picrosirius red stains were performed on sections before bright field imaging and automated ImageJ quantification of area containing blue fibrotic scar (A) with quantitative analysis (B) and red type I collagen (C) with quantitative analysis (D) for the respective stains. Treatment groups assessed were as follows: Sham (n=9), PBS (n=12), free SOD (n=10), NP-SOD (n=13). Significance represented as follows: *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.